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Image Search Results
Journal: BBA Clinical
Article Title: Glioblastomas with copy number gains in EGFR and RNF139 show increased expressions of carbonic anhydrase genes transformed by ENO1
doi: 10.1016/j.bbacli.2015.11.001
Figure Lengend Snippet: Quantified oncogenes in GB06. An example of multiplex ligation-dependent probe amplification (MLPA) results for 78 oncogenes in one glioblastoma. Its high level amplifications of PDGFRA and EGFR were readily detected as fluorescence signals from each of the 2 sets of probes used for each oncogene. The two-fold elevations in tumor versus normal brain were detected using mathematical comparisons described in . The red and blue lines represent tumor and normal DNA, respectively. Also, the inset at the top shows fluorescence in situ hybridization (FISH) signals for EGFR (red) in two cells of the frozen tissue sample used for MLPA. The nuclei stained blue with diaminophylindole (DAPI).
Article Snippet:
Techniques: Multiplex Assay, Ligation, Amplification, Fluorescence, In Situ Hybridization, Staining